Monoclonal antibodies (MAbs) are immunological active proteins, which bind specifically to certain cells or proteins. This will stimulate the immune system to attack those targets.
MAbs are very important for the treatment of different kinds of cancer and autoimmune diseases. Nowadays, a broad variety of therapeutical antibodies are availableon the market and several more are in research and development.
Due to their molecular weight of about 150 kDa, intact antibodies are usually analysed by IEX, SEC or HIC. In addition, RP methods are an easy tool as well. However, lack of sensitivity and resolution has been a hurdle in the past. With modern RP phases, addressing the requirements of these analytes, it is easy to find a suitable method.
Successful analysis in RP mode for MAbs is enhanced by employing a temperature stable (>60°C), widepore stationary phase.
This application note shows how to achieve robust chromatographic results for two commercially available MAbs: Adalimumab (e.g. Humira®) and Bevacizumab (Avastin®).
>> Download the full Application Note as PDF
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