Streamlined Sample Preparation of a Drugs of Abuse Panel in Human Nails Using ISOLUTE^® SLE+ Prior to UPLC-MS/MS Analysis

contributed by Biotage | 09/10/2019

The testing of alternative matrices in forensic and/or clinical toxicology is gaining popularity, partly due to less invasive means of collection. Matrices such as hair or nail can provide a more rounded picture of abstinence or abuse and associated timeframes. This application note describes the sample pre-treatment and subsequent extraction of 49 drugs of abuse from human nails, prior to LC/MS analysis.

The method utalizes Biotage^® Lysera for matrix micropulverisation, prior to direct transfer to clean up using ISOLUTE^® SLE+ supported liquid extraction products. Elimination of an evaporation step between the micropulverisation and supported liquid extraction clean up stages provides a streamlined procedure for nail extraction.

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Email*

Choose a password*

I have read and understand the Privacy Notice *

Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

Receive communication from The Cannabis Scientist I want to stay up to date with the Mass Spectrometry field I want to stay up to date with the Spectroscopy field

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.