Separation of Homologous Series of Technical Detergents with the Agilent 1290 Infinity 2D-LC Solution Coupled to an Evaporative Light Scattering Detector

contributed by Agilent Technologies | 02/18/2014

This Application Note shows the separation of a series of homologous non-ionic surfactants in a technical product with the Agilent 1290 Infinity 2D-LC Solution by combination of a HILIC column in the first dimension and a reversed phase column in the second dimension. Surfactants typically do not show activity in UV detection, hence, the Agilent 1260 Infinity ELSD was used. The visualization of the compounds detected by the ELSD is demonstrated with dedicated 2D-LC software.

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