Simultaneous Extraction of Aldosterone and Angiotensin I and II from Human Plasma Using EVOLUTE® EXPRESS ABN Prior to LC/MS Analysis

Introduction

This application note describes the simultaneous extraction of aldosterone and angiotensin from human plasma using the EVOLUTE^® EXPRESS ABN Solid Phase Extraction plates prior to LC-MS/MS analysis.

Plasma renin activity (PRA) determination is used in the diagnosis of primary aldosteronism, an important cause of secondary hypertension (high blood pressure). Concomitant measurement of both aldosterone and angiotensin (converted from renin over a time range) in the plasma sample is required. Traditionally the analysis of these species is performed separately. This application note describes the simultaneous extraction of aldosterone at low (< 25pg/mL) concentration and angiotensin I and II from plasma, with the potential to improve throughput and reduce time and costs.

The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 5% for all analytes using LC-MS/MS.

Figure 1. Structures of Aldosterone, Angiotensin I and Angiotensin II .

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