Elimination of an evaporation step between the micropulverization & supported liquid extraction clean up provides a streamlined procedure for hair extraction. Analytes quantified using LC-MS/MS.
This application note describes a streamlined procedure for sample pre-treatment and extraction of a panel of 49 drugs of abuse from human hair, using Biotage® Lysera for matrix micropulverization, prior to direct transfer to clean up using ISOLUTE® SLE supported liquid extraction.
Elimination of an evaporation step between the micropulverization and supported liquid extraction clean up stages provides a streamlined procedure for hair extraction. Analytes are quantified using LC-MS/MS.
The application note contains procedures optimized for the 400 μL capacity column format, and 200 μL and 400 μL supported liquid extraction plate formats, for higher throughput applications. The methodology delivers clean extracts and analyte recoveries mostly greater than 80% with RSDs lower than 10% for all analytes and LLOQ from 1 ng/mL.
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