The advantages of pressure-resistant, high-performance polymer columns in HPLC and LC-MS

contributed by Shodex | 11/25/2019

1. Popular, but no all-rounders: Silica gel columns and their limitations in liquid chromatography

Hydrophobic surface-modified silica gels of different selectivities and particle size distributions are by far the most important stationary phases for HPLC and U(H)PLC. Despite its popularity, silica gel has the disadvantage that a high pH mobile phase can dissolve the silica gel matrix. As a consequence, it is recommended to stay within a limited pH range of between 2 and 7 for the mobile phase when using a silica gel based separation column.

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