The Importance of Sample Diluent in HILIC

contributed by Ace | 11/20/2019

ABSTRACT

When working with Hydrophilic Interaction Liquid Chromatography (HILIC), the choice of sample diluent can have a profound impact on peak shape. An incorrect choice can lead to poor peak shape, peak splitting and unstable retention times. This ACE Knowledge Note discusses how a suitable choice can be determined during HILIC method development.

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