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Techniques & Tools Preparative/Process Chromatography, Liquid Chromatography, Technology

The Infinite Column

Peak recycling elongates the separation length of your column


Through peak recycling the user can benefit from a more efficient purification and reduced cost. This article describes when peak recycling is reasonable and the technical specification it requires. By using the length of the column bed multiple times, peak recycling basically elongates the separation length of your column. Most preparative chromatography users know the term but do not necessarily know in which cases this special solution is beneficial. During peak recycling, the peaks are led back onto the column after elution. Thereby, critical peak pairs that run together can be separated further with each cycle. The only requirement for peak recycling is an isocratic separation method since the composition of the eluent needs to stay constant during multiple repetition cycles.

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