Solvent is delivered to the open port sampling area through the outer pair of concentric tubes at ~250 μL/ min. Paired with the electrospray ion source of the expression Compact Mass Spectrometer, the solvent forms a meniscus at the open port before being drawn down the inner path into the ion source of the mass spectrometer under the Venturi effect of the nebulization gas. The control and fentanyl-spiked urine samples, contained in a pipette tip, are simply touched in sequence to the OPSI sampling port and results are obtained in < 30 sec.
The mass spectra of fentanyl and fentanyl-D5 (Figure 1) show protonated molecules at m/z 337.2 for fentanyl and 342.2 for fentanyl-D5. These ions are used in a Selected Ion Monitoring (SIM) method for the quantification of fentanyl in urine. The OPSI sample technique provides an effective, high-throughput assay for fentanyl screening applications.
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Figure 1: Mass spectra of Fentanyl & Fentanyl-D5 in control urine.>> Download the full Application Note as PDF
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