Like most websites The Analytical Scientist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.

Tracking Fate and Purge of Impurities and Calculating Carryover


The purpose of process development in pharma is to select and optimize a synthetic route to produce the active pharmaceutical ingredient (API) by the safest, cheapest, fastest, and cleanest (by green chemistry where possible) route, following both Good Laboratory Practice (GLP) and Quality by Design (QbD) principles. As with any synthetic process impurities are generated, and for API development it is mandated by regulatory authorities (e.g., Federal Drug Agency—FDA, European Medicines Agency—EMA, etc.) that impurities are tracked and identified above a certain threshold, while genotoxic and mutagenic impurities must be reported at any level (as stated in the ICH Q7 guideline1).

Route scouting data, in process development, is typically stored in electronic notebooks. Associated analytical information may be accessible as PDF images stored within an experiment record. Unfortunately, however, from the perspective of entity characterization and review, the analytical data is not dynamically linked with the individual stage(s) of the process route and is unsearchable and inaccessible.

Effective management of API development data, and particularly impurity tracking, is necessary for the development of an optimal impurity control strategy. In order to successfully track the fate and purge of impurities, many scientists currently gather LC/MS and LC area percent values for impurity entities in MS Excel® spreadsheets, in lieu of chemically intelligent software to manage this data. While spreadsheets are effective for handling and managing numerical data—helping formulate and understand relationships through sorting and plotting—they are a weak tool for relating chemical structures with the analytical spectra and chromatograms used to identify and characterize them. For example, Excel is unable to generate an impurity map from the synthetic route or calculate purge factor from the available analytical data.

Here, we discuss Luminata™—software designed to help project teams assemble synthetic routes, associated impurities, and all relevant analytical data for process development in a systematized and searchable manner. Luminata enables effective inter- and intra-departmental collaboration and automatically calculates purge factors directly from LC/MS and LC data. In this document, we focus on two manual and often tedious workflows: process optimization and fate & purge calculations.

Read the full article now

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE and always will be!

Login if you already created an account

Or register now - it’s free and always will be!

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine

Or Login as a Guest or via Social Media

Related Application Notes

Dedicated Cannabinoid Potency Testing Using the Agilent 1220 Infinity II LC System

| Contributed by Agilent Technologies

Techniques & Tools Gas Chromatography

What is thermal desorption?

| Contributed by Markes

Generic Analyses of Different Monoclonal Antibodies with RP-(U)HPLC

| Contributed by YMC


Send me the latest from The Analytical Scientist.

Sign up now

Related Articles

Techniques & Tools Capillary Electrophoresis

Gurus of Capillary Electrophoresis

| Cari Sänger-van de Griend, Hermann Wätzig, Michael Breadmore

Fields & Applications Pharma & Biopharma

Pharma Analysis: The Next Generation

| Jean-Luc Veuthey

Techniques & Tools Liquid Chromatography

Your Efficiency Challenge - Part IV

| Sponsored by Agilent Technologies

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine