Using the Statistical Compare and Fisher Ratio ChromaTOF Features to Define Variance Prior to Multivariate Analysis

Introduction

The "Statistical Compare" option available in ChromaTOF software allows the user to view statistical comparisons as a data processing step for groups of samples. The groups of samples are divided into different subsets or classes. ChromaTOF software aligns the data for the specified group of samples from the data processed peak tables. Upon completion of peak alignment, statistical information (such as minimum, maximum, average, relative standard deviation, etc.) on various peak properties (such as peak height, peak area, retention time, etc.) can be viewed in the Compound Table generated by Statistical Compare. The software will also compare statistical information from each class and between classes. Additionally, "Fisher Ratios" can be calculated from the Compound Table for each analyte. The Fisher ratio is a statistical calculation that can be used to discover the unknown chemical differences among known classes of complex samples. Statistical Compare results can also be exported as a .csv file and applied to third party software programs for supplemental data reduction such asmultivariate analysis.