A Case of Identity
A new sequencing method promises to streamline the characterization of bacteria
Jonathan James | | Quick Read
Successfully typing of bacteria at the species or strain level is not easy – fast, cost-effective methods like 16S ribosomal RNA gene sequencing lack accuracy, while technologies such as matrix-assisted laser desorption/ionization time-of-flight MS are more precise but remain expensive and laborious. Lukasz Krych and colleagues have developed an alternative: ON-rep-seq – an approach targeting specific fragments of the bacterial genome (1). “We’ve developed a system that assigns each bacterium a unique genetic barcode,” says Krych. “This allows us to run hundreds of samples simultaneously, drastically reducing the cost of analysis.”
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