Like most websites The Analytical Scientist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.
Fields & Applications Metabolomics & Lipidomics, Mass Spectrometry

Coprolite Parasite Insight

The increased spread of infectious disease during the Neolithic period is attributed to agricultural and population expansion as humans transitioned from hunter-gatherers to farmers and herders. Intestinal parasites in particular are thought to have adapted in response to altering human behavior at that time.

Marrisa Ledger and colleagues honed in on parasite infections in the village of Çatalhöyük, Turkey, by analyzing four samples of coprolite (fossilizes feces) from 6410-6150 BCE (1). The samples were first ground and analyzed by digital light microscopy to confirm the presence of parasite eggs; next, the total lipid extracts of the infected samples were subjected to GC-MS to determine levels of sterols (including those used as faecal biomarker compounds) and bile acids (produced by the organism of origin).

Two of the four samples tested positive for whipworm eggs (Trichuris sp.), most likely human whipworms (Trichuris trichuria), and lipid analysis indicated samples of human origin; sterol profiles were typical of omnivores (high coprostanol, low cholesterol and numerous phytosterols) and bile acid profiles were primarily deoxycholic acid, with few lithocholic acids. The results appear to provide the first evidence for intestinal parasite infection in this region at this time. Additional pelvic soil samples were also collected from nearby burial sites as a control – but all tested negative for parasite eggs.

The researchers plan to use the method to study additional compounds from coprolite samples in studies that go beyond simply confirming the species of origin. “We are developing a more holistic approach,” says investigator Ian Bull. “Many compounds derive from diet, so we hope that we can gain more information about the way people lived, as well as the commodities used.”

How much more information can be uncovered from such samples? The answer is, of course, tied inextricably to the team’s method, which has been evolving in line with instrumental innovation since the early 2000s. “Greater sensitivity is always appreciated but, certainly for us, better workflows and mining of data obtained from time-of-flight and Orbitrap MS systems is now key,” says Bull. “We can already collect vast amounts of information using such platforms – we just need to improve and simplify methods for us to interrogate such datasets. And that moves us very much in the direction of lipidomics and metabolomics.”

Enjoy our FREE content!

Log in or register to gain full unlimited access to all content on the The Analytical Scientist site. It’s FREE and always will be!


Or register now - it’s free and always will be!

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine

Or Login via Social Media

By clicking on any of the above social media links, you are agreeing to our Privacy Notice.

  1. ML Ledger et al., “Parasite Infection at the early farming community of Çatalhöyük”, Antiquity, 93, 573-587 (2019).

About the Author

Matthew Hallam

I've always wanted a career in which I could practice my creativity, even when I worked on the assembly line in a fish factory. At one time, I channeled this need into dance, drawing, poetry and fiction, and I still do most of these things. But, following completion of my MSc(Res) in Translational Oncology and time working in labs and as a Medical Writer for major pharmaceutical companies, I'm happy to find myself in a career that allows me to combine my creative side with my scientific mind as the Deputy Editor of The Analytical Scientist.


Send me the latest from The Analytical Scientist.

Sign up now

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine