Determination of Aflatoxin M1 in Milk
Fast and isocratic method with solid phase extraction, fluorescence detection and post column derivatization with an UVE photochemical reactor
Juliane Boettcher and Kate Monks |
sponsored by Knauer
Aflatoxins are the best known group of mycotoxins (naturally occurring mushroom poisons) and can accumulate on crops during storage of agricultural products, especially under warm and humid conditions. The maximum aflatoxin M1 level set by the U.S. Food and Drug Administration and European Commission is 0.5 µg/L. The application describes a fast and isocratic method applicable for the required detection limit of aflatoxin M1.
First, the analytical method was developed using a standard solution: standard concentration of 1 µg/mL aflatoxin M1 with fluorescence detection and post column derivatization using the UVE photochemical reactor. To make sure that the legal limit value is detectable, a milk sample was spiked with aflatoxin M1 to a concentration of 0.5 µg/L and pretreated with online solid-phase extraction (SPE). Figure 1 shows an overlay of the spiked milk sample after sample preparation and the aflatoxin M1 standard. Although matrix effects occur through SPE pretreatment, it was possible to quantify aflatoxin M1 in the measured milk sample spiked down to 0.5 µg/L. For sample pretreatment the following SPE procedure was conducted: 20 mL of the spiked milk was diluted with 30 mL distilled water. A CHROMABOND® C18 ec SPE column was conditioned with 10 mL water and 10 mL n-hexane. Afterwards, the column was dried for 10-20 min at 50°C or overnight at ambient temperature. After drying, the sample was eluted with 3 mL acetonitrile.
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