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Fields & Applications Sample Preparation, Food, Beverage & Agriculture

Extraction of Aflatoxins and Ochratoxin from Dried Chili Using ISOLUTE® Myco Prior to LC-MS/MS Analysis

sponsored by Biotage

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This application note describes a solid phase extraction (SPE) protocol for the extraction of a range of mycotoxins from dried chili (pimiento) using ISOLUTE® Myco with LC-MS/MS analysis. The method described achieves high recoveries of relevant mycotoxins from dried chili (pimiento) with %RSDs and LOQs that meet the requirements set in European Union regulations for measurement of these analytes.

By Adam Senior, Biotage GB Ltd, Hengoed, UK

Analytes: Aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A

Sample Preparation Procedure

Format: ISOLUTE® Myco 60 mg/3 mL Columns (Tabless), part number 150-0006-BG
Sample processing: Grind the sample (50 g) with a burr-grinder or equivalent device. Store ground sample in a sealed container at room temperature until required.
Extraction: Mix the ground sample (5 g) with 80% acetonitrile (aq) (20 mL). Place the sample pre-treatment tube on a shaking table for 30 mins. Transfer the extract to a 50 mL centrifuge tube and centrifuge at 4000 g for 10 minutes.
Dilution: Take the supernatant (2 mL), transfer to a new 50 mL centrifuge tube and dilute with water (32 mL). Centrifuge diluted extract at 4000 g for a further 10 minutes.

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