Fields & Applications Spectroscopy, Food, Beverage & Agriculture, Pharma & Biopharma

GPC/SEC-MALLS analysis of Hyaluronic Acid

Hyaluronic acid is a polymer of disaccharides and can have up to 25,000 disaccharide repeat units. The molar mass range is from 5000 Da to 20 000 000 Da. Hyaluronic acid is mainly used in medical and cosmetic applications.

04/16/2013

sponsored by PSS

GPC/SEC is the method of choice for measuring the molar mass distribution of hyaluronic acid. True molar masses can be obtained when on-line multi angle laser light scattering (MALLS) is used in combination with a refractive index detector. The refractive index increment (dn/dc) for light scattering data evaluation can be determined on-line, offline using dedicated instrumentation or taken from literature.

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