Fields & Applications Food, Beverage & Agriculture

Sensitive analysis of the lactose content of lactose-free labelled products using HPAEC-PAD

10/20/2015

sponsored by Knauer

The market for lactose-free products is growing rapidly and constantly and Europe is a worldwide leader in the lactose-free market. Between 2012 and 2016 the sales of lactosefree products are expected to increase by 75 %.^1,2 Studies additionally state that customers who are lactose intolerant or believe they are, will pay a big premium for the right product.¹ From these statements it becomes obvious that a huge demand for lactose-free products is existing in the industry.

An HPLC method that easily reaches the required LOD by using High-Performance Anion- Exchange Chromatography coupled to pulsed amperometric detection (HPAEC-PAD) on a KNAUER AZURA^® Analytical HPLC system coupled to the DECADE II electrochemical detector is presented. Besides lactose, also sucrose and glucose were analyzed to prove the method's ability to differentiate several sugars.

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