Small-footprint Absorbance System for Characterizing Heme Proteins
Compact Spark spectral sensor measures Vis-NIR absorbance of hemoglobin – and more
Yvette Mattley |
sponsored by Ocean Optics
In this application note, we focus on the visible response of the heme group using the compact Spark spectral sensor to measure absorbance spectra for the heme proteins hemoglobin, myoglobin and cytochrome c.
The heme groups found in metalloproteins generate absorbance bands that vary based on the state of the heme group. These absorbance changes make UV-Vis spectroscopy a very powerful tool for studying heme proteins. Absorbance measurements can be used to study changes to critical protein parameters like protein conformation and to provide information on the binding and oxidation state of the heme prosthetic group.
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