Subscribe to Newsletter
Techniques & Tools Liquid Chromatography

Change on the Horizon

From escalating concerns about pollutants in the environment and contamination in food, to the need for multiple attribute measurements in biopharma or chemical products, there is an increasing demand for separation power. Chromatography and (ion mobility [IM])-mass spectrometry (MS) are powerful technologies individually but their hyphenation strongly amplifies the power of the analytical platform. Multidimensional LC boosts separation power even further and allows us to make several measurements in a single analysis. Good examples can be found in the biopharmaceutical industry, where we may want to determine concentration of the API, aggregation, charge heterogeneity, structural integrity and more. For instance, Protein-A is often coupled with size exclusion chromatography (SEC) for the analysis of monoclonal antibodies (mAbs).

Another trend is to couple two methods with different selectivity for the analytes of interest. Co-eluting compounds from the first dimension are simply sent into second dimension to be resolved. Thus, the development effort for a 1D method, which for complicated samples can be time-consuming, can be dramatically reduced or even eliminated. A good example is the work of Dwight Stoll (Gustavus Adolphus College) on selective comprehensive 2D-LC (sLC×LC or multiple heart-cutting), which decouples 1D and 2D timescales by using multiple loops for sample storage of several sections across the 1D-chromatogram. The transition from full to selective comprehensive 2D-LC has been a major driver in the increasing popularity of the technique.

Right now, system hardware is robust, method setup is simplified by clever software solutions, and so 2D chromatography is becoming widely accepted. In contrast to those early days, when only a few used the technology, the ongoing exchange of ideas and experience within a growing 2D-LC community continuously amplify our knowledge, driving us all forward.

So, what’s next? Future developments should focus on supporting method development as shown by Peter Schoenmakers’ group at the University of Amsterdam (1). In particular, better solutions for data analysis are required when extremely complex samples (such as omics samples) are analyzed using everything on the bench (for example, comprehensive 2D-LC coupled to IMS/QTOF).  We believe that forthcoming advances in these areas – and wider recognition of the incredible separation power achievable – will push 2D-LC into more routine applications.

Want to read more about "The Coming of Age of 2D-LC"? Click here.

Receive content, products, events as well as relevant industry updates from The Analytical Scientist and its sponsors.
Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

  1. R Pirok et al., “Program for the interpretive optimization of two-dimensional resolution”, J Chromatogr A, 1450, 29–37 (2016).
About the Authors
Monika Dittmann

Monika Dittmann is Principal Scientist R&D at Agilent Technologies, Germany.

Stephan Buckenmaier

Senior Research Scientist at Agilent Technologies

Related Application Notes
Reproducible oligonucleotide IEX analyses from the very first injection!

| Contributed by YMC

Separation of plasmid isoforms using BioPro HIC BF

| Contributed by YMC

Safer AAV Analysis with Non-toxic AEX Method

| Contributed by Tosoh

Related Product Profiles
The fine Art of Method Development

| Contributed by Shimadzu Europa

Higher Peaks – Clearly.

| Contributed by Shimadzu Europa

Compact with countless benefits

| Contributed by Shimadzu Europa

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:
  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine