Subscribe to Newsletter
Techniques & Tools Mass Spectrometry, Translational Science

Lipid Locators

A cross-border collaborative group has developed a microarray/MS-based method that promises simplified lipid profiling in tissues and cells (1). We sat down with two of the team – Gabriel Barreda-Gómez and José Andrés Fernández González – to find out more.

What was the inspiration for your work?

Our objective was to develop a novel method capable of determining the lipid phenotype of cells, tissues and organs. We’re particularly interested in the brain, so we went in search of an approach that would allow us to identify specific cell subtypes in brain tissue, as well as determine their proportions and characterize the effect of cancer on the composition of their membranes.

How does the method work?

We make use of two complementary technologies: microarrays and matrix-assisted laser desorption/ionization (MALDI)-MS. The microarrays can hold membrane homogenates, lipid extracts or intact cells, spotted on the surface of a microscope slide using a piezoelectric robot to minimize the volume of sample required. We’re then able to utilize MALDI-MS to generate our data because we use a sublimator to ensure each spot on the array is covered with a similar amount of matrix. Our approach also allows us to combine lipid profiling with other experiments, such as protein affinity or autoradiography, as the membrane proteins in our samples remain fully functional.

Use of microarrays to uncover patterns in lipid expression across sections of rat brain samples.

What are your aspirations?

The technology is well-suited to the design of diagnostic tests based on lipid biomarkers. One possible application is tumor screening; the growth of malignant cells requires a great synthesis of new membranes so we would expect to see significant changes in the lipid fingerprint of a tissue, even in the early stages of cancer. We envisage our approach complementing the current gold standard (traditional histology) and hope it might go some way to reducing the growing pressure placed on hospital pathology departments.

Receive content, products, events as well as relevant industry updates from The Analytical Scientist and its sponsors.
Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

  1. R Fernandes et al., “Microarray and mass spectrometry-based methodology for lipid profiling of tissues and cell cultures”, Anal Chem, 24, 15967 (2019). DOI: 10.1021/acs.analchem.9b04529
About the Author
Jonathan James

Having thrown myself into various science communication activities whilst studying science at University, I soon came to realize where my passions truly lie; outside the laboratory, telling the stories of the remarkable men and women conducting groundbreaking research. Now, at Texere, I have the opportunity to do just that.

Related Application Notes
Expand the Typical Breath Analysis into a Dynamic real-time Analysis of Metabolic Changes

| Contributed by Plasmion GmbH

Ultra-sensitive nitrosamine detection via H2: speed up routine analysis up to 50%

| Contributed by Plasmion GmbH

Best practices for sample preparation for Mass Spectrometry

| Contributed by Bertin Technologies

Related Product Profiles
Higher Peaks – Clearly.

| Contributed by Shimadzu Europa

Compact with countless benefits

| Contributed by Shimadzu Europa

The fine Art of Method Development

| Contributed by Shimadzu Europa

Most Popular
Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:
  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine