Carbohydrate Analysis in Instant Coffee According to Adapted ISO Method 11292:1995

07/09/2018

In this application note the analysis of carbohydrates in instant coffee, following the ISO 11292:1995 method, was evaluated using the ALEXYS carbohydrates analyzer. The method is based on separation of carbohydrates by High Performance Anion Exchange Chromatography followed by Pulsed Amperometric Detection (HPAEC-PAD) under standard bore conditions. An adapted method was evaluated, which uses narrow-bore conditions and a column with smaller particle size. In comparison with the standard method, the adapted method resulted in 50% shorter analysis times and 90% lower solvent use (‘greener’ solution). Both standard bore and narrow-bore methods provide excellent sensitivity, linearity and reproducibility, and are suitable for the routine analysis of carbohydrates in coffee applications using the ALEXYS carbohydrates analyzer.

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