Computer aided method development using in-silico computer modelling and 6 columns with unique selectivities

INTRODUCTION

Reversed-phase method development can be a lengthy process which can tie up valuable resources in an analytical laboratory. The process followed and success obtained can vary depending upon experience, resources and available time. Often, a trial and error (or one factor at a time) approach is adopted, where parameters are adjusted and decisions made according to the analytical results obtained for each iterative step. This approach can produce acceptable separations, but it may fail to identify the most suitable method, i.e. the most robust or the most cost effective (e.g. fastest) method.