Cookies

Like most websites The Analytical Scientist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.
Fields & Applications Forensics, Sample Preparation, Liquid Chromatography, Mass Spectrometry

Drugs of Abuse in Oral Fluids: Automated SPE Extraction and LC/MS/MS Determination using a Robotic Autosampler

Abstract

Solid phase extraction (SPE) is one of the sample preparation methods most widely used by chromatographers, as can be seen from the large number of SPE methods found in the literature. Typically, a liquid sample is passed across an adsorbent bed to retain and concentrate target analytes and eliminate interference from the sample matrix. Alternatively, the adsorbent can be used to retain interferences while allowing the target analytes to pass through. Manual SPE cartridge formats can vary from disks to individual cartridges with various volumes of sorbent to 96-well plates. However, solid phase extraction can be tedious and time consuming when performed manually and there is increasing demand for automation of SPE methods.

The MPS roboticpro is a highly effi cient LC or GC autosampler with extended robotic functionality. The MPS roboticpro provides reliable processing of complex tasks including automation of SPE procedures. Syringe holders and syringes are integrated in special syringe modules, which can be exchanged automatically within a running sequence for maximum flexibility. The system is controlled in a simple and effi cient manner using the proven GERSTEL MAESTRO software. In this study, we show that an existing SPE method using ITSP cartridges [1] can be automated using the MPS roboticpro autosampler under MAESTRO control. An example of a solid phase extraction method illustrating the determination of drugs of abuse in oral fluid is shown.

Enjoy our FREE content!

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE and always will be!

Login if you already created an account

Or register now - it’s free and always will be!

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine
Register

Or Login as a Guest or via Social Media

Newsletter

Send me the latest from The Analytical Scientist.

Sign up now

Related Articles

Fields & Applications Forensics

Deathly Metal

| Joanna Cummings

Business & Education Technology

A Philadelphia Story

| Joanna Cummings

Fields & Applications Forensics

Finding Narco

| Joanna Cummings

Most Popular

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine

Register