Extraction of 45 Multi Class Drugs of Abuse from Hydrolyzed Urine

contributed by Biotage | 06/24/2013

This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of various drugs of abuse from hydrolyzed urine prior to LC-MS/MS analysis.

The method described in this application note achieves high reproducible recoveries for a wide range of drugs of abuse and metabolites from hydrolyzed urine. Hydrolyzed urine was extracted using the ISOLUTE SLE+ 200 supported liquid extraction plate and 1 mL column formats using 100 and 500 μL sample volumes, respectively. Sub ng/mL levels were achieved for all analytes using 100 μL of urine on the fixed well plate format. ISOLUTE® SLE+ products provide clean, rapid, robust, efficient, high throughput and automatable extraction solutions for a wide range of analytes.

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