Extraction of Synthetic Cannabinoids (SPICE) from Oral Fluid using ISOLUTE® SLE+

Summary

This application note describes the extraction of a range of SPICE drugs and metabolites from neat oral fluid and oral fluid from a commercial collection kit using ISOLUTE® SLE+ in both 96-well plate and column formats.

Introduction

Synthetic cannabinoids have become an increasing problem as an ever changing target for detection during drug screening. The need for quick, non-invasive sampling has been desired by law enforcement as a way of detecting illicit drug use. The collection of oral fluid addresses this need. A semi-automated extraction workflow process was developed for SPICE drugs fortified in oral fluid samples prior to LC-MS/MS. The method was facilitated using Biotage PRESSURE+ 96 and PRESSURE+ 48 Positive Pressure Manifolds for 400 μL sample capacity 96-well plates and columns, respectively. Oral fluid was collected both as a neat solution, and using a commercially available Intercept® (Orasure) kit.

ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation time.