Gradient Method Translation Using the ACE LC Translator

Abstract

Translating gradient LC methods from one column dimension to another is common, but maintaining the separation selectivity and peak resolution can be challenging. For the most reliable translation of gradient methods between column formats, careful consideration of fundamental chromatographic principles is required. Omitting key parameters in calculations can lead to detrimental changes in selectivity or peak resolution of the translated gradient method. The ACE LC Translator is based upon fundamental principles and includes all key parameters and calculations required for an accurate gradient method translation process.