Method development HILIC white paper
An intelligent solution for hydrophilic interaction liquid chromatography method development
contributed by VWR |
What is Hydrophilic Interaction Liquid Chromatography (HILIC)?
Hydrophilic Interaction Liquid Chromatography (HILIC) has evolved into a powerful chromatographic technique for the retention and separation of hydrophilic neutral and polar compounds, which are often difficult to retain by Reversed-Phase Liquid Chromatography (RPLC). HILIC utilises a polar stationary phase combined with an organic/aqueous mobile phase , typically containing a high percentage of the organic component (~>60% acetonitrile). In HILIC, water is the strong solvent and unlike RPLC, increasing the percentage of water in the mobile phase decreases analyte retention (Figure 1).
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