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Method development HILIC white paper

What is Hydrophilic Interaction Liquid Chromatography (HILIC)?

Hydrophilic Interaction Liquid Chromatography (HILIC) has evolved into a powerful chromatographic technique for the retention and separation of hydrophilic neutral and polar compounds, which are often difficult to retain by Reversed-Phase Liquid Chromatography (RPLC). HILIC utilises a polar stationary phase combined with an organic/aqueous mobile phase [1], typically containing a high percentage of the organic component (~>60% acetonitrile). In HILIC, water is the strong solvent and unlike RPLC, increasing the percentage of water in the mobile phase decreases analyte retention (Figure 1).

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