Sensitive and Accurate Quantification of Isoprostane Oxidative Biomarkers

Increasing selectivity with high-resolution multiple reaction monitoring (MRMHR) improves the signal-to-noise ratio for better accuracy, dynamic range, and LLOQ when quantitating isobaric prostaglandin-D2α isoforms

Key challenges of isoprostane biomarker assays

• Lack of sensitivity – Quantification is poorly reproducible at low picogram levels in complex biological matrices.
• Overlapping interferences – Assay selectivity is hampered by interfering, co-eluting peaks.
• Multicomponent analysis in single assay –Single isomer measurement is a poor indicator of oxidative stress due to rapid degradation and variable isomer formation.
• Substandard data quality – Precision and accuracy are compromised at very low biomarker levels, giving results below accepted bioanalytical standards.