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UHPLC Analysis of St John’s Wort using Column Coupling

Introduction

Many natural products have historically proved valuable for the treatment of a wide range of medical conditions. The quantitative and qualitative analysis of natural products is important to confirm authenticity, origin and quality control. The analysis of natural products by liquid chromatography is challenging due to the highly complex nature of samples, which may potentially contain hundreds of structurally diverse individual components. Faced with such samples, it is useful to use chromatographic techniques that can deliver exceptionally high resolving power.

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