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Fields & Applications Liquid Chromatography, Food, Beverage & Agriculture

Determination of Lactose in Lactose-free Products Using HPAEC-PAD

sponsored by Knauer

Special methods and systems are needed because classical determination of sugars in food products with RI detection is far too insensitive for this case. An HPLC method that easily reaches the required limit of detection (LOD) by using high-performance anion-exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD) is presented. After calibration, several food products were analysed to determine the lactose content.

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About the Author
Mareike Margraf and Dr. Kate Monks
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