Determination of Lactose in Lactose-free Products Using HPAEC-PAD
In many European countries, the limit of lactose in lactose-free labelled products has been lowered from 100 to 10 mg/100 g product in recent years (1).
Mareike Margraf and Dr. Kate Monks |
sponsored by Knauer
Special methods and systems are needed because classical determination of sugars in food products with RI detection is far too insensitive for this case. An HPLC method that easily reaches the required limit of detection (LOD) by using high-performance anion-exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD) is presented. After calibration, several food products were analysed to determine the lactose content.
