Techniques & Tools Liquid Chromatography

Introducing the latest in UHPLC technology

Peptide mapping method uses a novel high resolution column with new UHPLC technology.

07/25/2013

sponsored by VWR

Peptide Mapping uses UHPLC to check the constituent amino acids by comparing the pattern of the peptide fragment chromatogram after the enzyme digestion of protein. Recently, this method has also been applied to quality control testing of bio-pharmaceutical therapies.

As a result, it is important that the chromatography is of high resolution and high speed with very low variability. Here, the high resolution, high speed measurement result using the ChromasterUltraRs is introduced.BSA digest was used as model sample, and a comparison was made between conventional and high resolution columns. The peak capacity (4σ) for each column was then compared.

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