Cookies

Like most websites The Analytical Scientist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.
Techniques & Tools Liquid Chromatography

Landmark Literature: 2015 (part3)

Practically and Fundamentally Excellent

By Peter Schoenmakers, Education Director COAST; Editor, Journal of Chromatography A; Professor, Analytical Chemistry/Forensic Science, University of Amsterdam, The Netherlands

A recent paper by Fabrice Gritti, Thomas McDonald and Martin Gilar struck me for several reasons. Firstly, it is an immensely important publication for liquid chromatographers. Such steadfast folk tend to strive for high efficiencies and excellent separations by making or using better columns with very small and uniform particles, perfectly homogeneous monoliths, and so on. But, unfortunately, they forget that long or poorly designed connectors and extra-column apparatus cause them to lose what they aim to gain – a problem the paper attempts to correct. In addition, the paper describes ingenious and original methods. It is a practical liquid chromatography (LC) paper, with theory supporting the experiments that aim to capture the causes of “misdemeanors” by short pieces of connection tubing. The results from the tedious experiments are consistent and rather surprising, the latter being mandatory for an interesting paper.

The paper contains some recommendations that will not surprise you, such as using the narrowest possible tubing and elevated temperatures, and voices a suggestion that we hear with increasing frequency, “Shouldn’t we all convert to supercritical-fluid chromatography (SFC)?” When we replace conventional water-acetonitrile mixtures in LC with mixtures of carbon dioxide and methanol for SFC, it is possible to perform faster separations (which we already know) and extra-column band broadening may be less of a threat (which is more remarkable than it seems – see SFC Star). Therefore, it will be interesting to see whether SFC will begin to catch up with LC. SFC has fundamental properties on its side (faster diffusion, lower viscosity), but aqueous-organic mixtures have proven to be fantastic eluents for all kinds of samples.

Read the full article now

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE and always will be!

Login

Or register now - it’s free and always will be!

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine
Register

Or Login via Social Media

By clicking on any of the above social media links, you are agreeing to our Privacy Notice.

About the Authors

Pat Sandra

Pat Sandra is Emeritus Professor of Organic Chemistry at Ghent University, and Founder and President of the Research Institute for Chromatography (RIC), Kortrijk, Belgium. “Through the activities of RIC, I got in touch with the real analytical needs of the industry and found we could help in providing solutions that are economically relevant. Moreover, it allowed me to keep my best PhD students around me, which resulted in high scientific output in a non-academic environment,” he says.


Peter Schoenmakers

Peter is education director COAST; editor, Journal of Chromatography A; and, professor, Analytical Chemistry/Forensic Science, van't Hoff Institute for Molecular Sciences, University of Amsterdam, The Netherlands. He obtained a masters in chemical engineering in 1977 and PhD in 1981, which included a research project on reversed-phase liquid chromatography. After fulfilling his military service, he had a career in industrial R&D, first at Philips and subsequently at Shell. He has been a fulltime professor since 2002 and now holds the chair of Analytical Chemistry, including its applications in Forensic Science. “My research interests include analytical separations (chromatography and mass spectrometry) and their applications to large molecules. A specific focus in recent years has been on the theory and application of comprehensive two-dimensional liquid chromatography.” Peter is active in the organization of several successful symposium series. He is a member of the permanent scientific committee of the HPLC series of conferences and chairman of the symposia on the Separation and Characterization of Natural and Synthetic Macromolecules (SCM) Hyphenated Techniques in Chromatography (HTC) and HPLC2013 in Amsterdam. He has been editor of the Journal of Chromatography A since 2003.

Newsletter

Send me the latest from The Analytical Scientist.

Sign up now

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine

Register