Cookies

Like most websites The Analytical Scientist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.
Techniques & Tools Liquid Chromatography, Mass Spectrometry, Pharma & Biopharma

Wanted: Native Protein LC-MS

The biologics field is booming and showing no signs of slowing down. As the biotech industry has developed, it has adopted advanced engineering techniques to produce antibody-drug conjugates (ADCs), bispecific monoclonal antibodies, new types of fusion protein scaffolds – and the products are becoming increasingly complex. Yet complexity is no excuse for compromise when it comes to patient safety or lot-to-lot reproducibility, so analytical assays must be up to the challenge of converting complicated biomolecular puzzles into tractable, well-characterized molecules.

There is no one-size-fits-all method for addressing every characterization challenge presented by these new modalities, but it is reasonable to suggest that LC and MS will play a central role. Advances in LC-MS have made it easier than ever to confirm recombinant protein sequences and to investigate product-related impurities. However, LC separations coupled to MS detection have relied largely on denaturing conditions that afford sensitive detection but also restrict our ability to investigate protein conformations and interactions. In short, techniques that couple native separations to MS analysis are needed.

Read the full article now

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE and always will be!

Login

Or register now - it’s free and always will be!

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine
Register

Or Login via Social Media

By clicking on any of the above social media links, you are agreeing to our Privacy Notice.

About the Author

Matthew A. Lauber

Waters Corporation, Milford, Massachusetts, USA.

Newsletter

Send me the latest from The Analytical Scientist.

Sign up now

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine

Register