Intrinsic viscosity measurements on sRAGE protein
In this application note, intrinsic viscosity measurements were performed on the sRAGE protein to establish whether the detector was able to detect and characterize the conformational change induced by the presence of calcium. Light scattering molecular weight measurements were also performed. Data are also presented showing that the conformation of sRAGE changes in the presence and absence of calcium.
sponsored by Malvern Panalytical
Work performed in conjunction with the Molecular Structure Group, IBMC, University of Porto, Portugal
Introduction
Size-exclusion chromatography (SEC) is a commonly used tool in the biological sciences. Typically, it is used to measure the molecular weight of an unknown sample by referencing the elution time of the unknown against that of standards of known molecular weight. This can be done with a single concentration detector such as UV or refractive index (RI). Advanced multi-detector SEC systems now include up to four detectors in series for maximum sensitivity; including UV, RI, light scattering and intrinsic viscosity detectors. Light scattering detectors allow the measurement of molecular weight without the need for column calibration.
