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Intrinsic viscosity measurements on sRAGE protein

sponsored by Malvern Panalytical

Work performed in conjunction with the Molecular Structure Group, IBMC, University of Porto, Portugal

Introduction

Size-exclusion chromatography (SEC) is a commonly used tool in the biological sciences. Typically, it is used to measure the molecular weight of an unknown sample by referencing the elution time of the unknown against that of standards of known molecular weight. This can be done with a single concentration detector such as UV or refractive index (RI). Advanced multi-detector SEC systems now include up to four detectors in series for maximum sensitivity; including UV, RI, light scattering and intrinsic viscosity detectors. Light scattering detectors allow the measurement of molecular weight without the need for column calibration.

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