Taming the Third Dimension

In an ambitious bid to boost separation performance, spatial 3D liquid chromatography prototypes aim to leave an impression.

By Jelle De Vos and Sebastiaan Eeltink

March 2017

The problem

The resolving power provided by contemporary 1D- and 2D-LC technology is insufficient to tackle the complex sample mixtures encountered in modern life-science studies, where each sample may contain hundreds of thousands of proteins. Furthermore, when high-peak capacity separations can be realized, it is often only achieved at the expense of very long analysis times, making the technology intrinsically unsuitable for biomarker discovery and validation studies.