Subscribe to Newsletter
Techniques & Tools Liquid Chromatography, Preparative/Process Chromatography

Method Robustness and Transferability for Modern Size-Exclusion Methods

sponsored by Phenomenex

Register now

Size exclusion chromatography (SEC) is a well-established analytical method for quantitative and qualitative aggregate analysis of monoclonal antibodies (mAbs) and other related biotherapeutics. However, because SEC is a non-adsorptive separation modality, it may be unclear as to which method parameters to adjust for the optimal separation of monomer, aggregate, and fragment.

In this presentation, we discuss SEC method experimental design space. Column selection, mobile phase, flowrate, and injection volume are all discussed to ensure optimal separation of monomer, aggregate, and fragment of mAbs and other mAb formats.

Webinar Learning Objectives:

  • Learn about a breakthrough in SEC column development to improve method robustness for monoclonal antibodies and related recombinant proteins
  • Understand how column choice‑including exclusion limit, particle size, and column length may affect chromatographic performance
  • Determine a practical mobile phase screen to optimize mAb aggregate recovery, fragment separation and understand when organic solvent should be used and how other co-solvents may be beneficial in improving peak shape and recovery

Register now

Visit our Webinar Hub

Receive content, products, events as well as relevant industry updates from The Analytical Scientist and its sponsors.
Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

Related Application Notes
Separation of plasmid isoforms using BioPro HIC BF

| Contributed by YMC

Safer AAV Analysis with Non-toxic AEX Method

| Contributed by Tosoh

Non-targeted profiling of polar metabolites in human plasma

| Contributed by YMC

Related Product Profiles
The fine Art of Method Development

| Contributed by Shimadzu Europa

Higher Peaks – Clearly.

| Contributed by Shimadzu Europa

Compact with countless benefits

| Contributed by Shimadzu Europa

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:
  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine