5 Key Takeaways

• 1

  A label-free single-cell proteomics workflow enables detailed characterization of cell states in complex vascular tissue.

• 2

  Researchers profiled nearly 5,000 individual cells from mouse aortic tissue, quantifying around 500 proteins per cell.

• 3

  Clustering identified major aortic cell types and distinct smooth muscle cell phenotypes, with some linked to Marfan syndrome.

• 4

  Endothelial cells in Marfan mice showed reduced adhesion proteins and increased smooth muscle-associated proteins.

• 5

  Integrating transcriptomic and proteomic data revealed additional clusters, highlighting potential disease markers.