Objective:

To explore the evolution, challenges, and future directions of non-target screening (NTS) in various scientific fields, including metabolomics, environmental science, and food safety.

Key Findings:

• NTS has become integral in various fields, including metabolomics, environmental science, and food safety, facilitating the discovery of unknown compounds.
• Data handling and interpretation are critical components of NTS due to the vast amount of data generated, necessitating advanced analytical tools.
• Collaboration across disciplines enhances the application and understanding of NTS, leading to innovative solutions in complex analytical challenges.

Interpretation:

NTS is evolving with advancements in data management and analytical techniques, highlighting the need for interdisciplinary collaboration to address complex analytical challenges and improve real-world applications.

Limitations:

• The reliance on machine-readable data can limit accessibility for some laboratories, but ongoing efforts aim to develop more user-friendly data management systems.
• The complexity of data interpretation may require specialized training and resources, prompting initiatives for broader educational outreach.

Conclusion:

NTS is a rapidly advancing field with significant potential across various scientific domains, necessitating ongoing collaboration and innovation.