5 Key Takeaways

• 1

  A new protocol allows simultaneous extraction of metabolites and lipids from a single dried blood spot for two omics analyses.

• 2

  The method utilizes a modified Folch extraction with a 2:1 chloroform and methanol mixture to separate metabolites and lipids.

• 3

  The workflow includes reversed-phase liquid chromatography-tandem mass spectrometry for separate analysis of metabolites and lipids.

• 4

  Data processing employs open-source tools Mzmine for metabolite features and LipidMatch for lipid data.

• 5

  Results are semiquantitative, allowing comparison of peak heights or areas, but absolute concentrations require further validation.