Advanced Data Acquisition and Data Processing Workflows to Identify, Quantify and Confirm Pesticide Residues

contributed by SCIEX | 03/08/2015

Pesticides are widely used in agriculture to protect crops and to improve efficiency of production. Pesticide residues may pose a potential threat to human health. Modern analytical techniques, such as QuEChERS extraction followed by LC-MS/MS, allow screening for pesticides in a variety of food matrices.^1-3

Here we present a new and powerful workflow to identify, quantify and confirm the presence of 400 pesticides utilizing generic QuEChERS extraction and LC-MS/MS analysis with the AB SCIEX QTRAP^® 6500 system using the Scheduled MRM™ Pro algorithm and Information Dependent Acquisition (IDA) of full scan MS/MS spectra. High confidence in identification and confirmation was achieved by automatically calculating the ratio of quantifier and qualifier ions and searching MS/MS spectral libraries in MultiQuant™ and MasterView™ software. Qualitative method performance was verified using guideline SANCO/12571/2013 guideline.⁴

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