Quantitation of Nicotinic Acid and Nicotinamide in Human Plasma

contributed by Phenomenex | 06/13/2013

Nicotinic acid and nicotinamide were extracted from human plasma by performing a rapid protein precipitation using Impact Protein Precipitation Plates followed by HPLC analysis using a Gemini 3 μm C18 100 x 4.6 mm HPLC column and positive polarity ESI LC/MS/MS system. Impact technology offers easy, fast protein removal while providing maximized recovery of the target analytes. The Gemini 3 μm C18 HPLC column produced excellent chromatographic resolution, sensitivity, and high peak capacities.

Niacin (nicotinic acid) is a water-soluble vitamin that is also referred to as vitamin B3. Nicotinamide (nicotinic acid amide) is the derivative of niacin that is incorporated into the coenzymes nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). The nicotinamide moiety of NAD and NADP serves as an electron acceptor or donor in biological oxidation-reduction reactions catalyzed by several hundred different enzymes. Both nicotinic acid and nicotinamide are absorbed from the normal diet.

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