TSKgel High Temperature GPC Column Durability

Introduction

In gel permeation chromatography (GPC), just like all chromatography modes, the heart of the separation lies in the quality, applicability and selectivity, or resolution, of the column. The selectivity of a GPC column is based on the ability of the pores in the column packing material to differentiate between species of varying hydrodynamic volume. The pores of the packing material within a GPC column are sampled by the analytes as they travel through the column in a size dependent manner. Due to their size, the larger components of the analyte sample either a smaller number of pores or, within a given pore, a smaller pore volume than the smaller components of the analyte, thus the larger components elute from the column prior to the smaller components.