UHPLC Column Protection Dramatically Extends UHPLC Column Performance and Lifetime

Introduction

UHPLC columns can significantly improve chromatographic separations, but they also present unique challenges. Once the UHPLC system components are optimized, perhaps your greatest concern is protecting the column from the damaging effects of microparticulates and sample contaminants.

An ultra-high performance column protection system, specifically designed for UHPLC systems using sub-2 μm and core-shell particle columns, can be used to extend column lifetime (saving both money and time through less frequent column replacement), while minimizing system troubleshooting and downtime.

Results and Discussion

It is well known that UHPLC systems and columns require higher levels of care and attention than traditional HPLC in order to reap their full ultra-high chromatographic performance benefits. Once system components are optimized, chromatographic cleanliness is vital to maintain UHPLC performance, and column protection is compulsory. Unprotected columns may suffer from reduced performance and lifetime, and may lead to an increased need for system troubleshooting and/or downtime.