5 Key Takeaways

• 1

  A new workflow using fluorine tracking could replace radioactive labeling in drug metabolism studies.

• 2

  The method employs ICP-MS to achieve sensitive, quantitative metabolite profiling without radioactivity.

• 3

  Detection limits below 1 ng on-column were achieved by combining capillary LC with tandem ICP-MS.

• 4

  The workflow demonstrated comparable performance to traditional ¹⁴C detection in both in vitro and in vivo samples.

• 5

  Fluorine's presence in many drugs and minimal background in biological matrices make it an ideal target.