5 Key Takeaways

• 1

  Researchers at Genentech transformed hydrophobic interaction chromatography (HIC) into a real-time monitoring tool for antibody-drug conjugates (ADCs).

• 2

  By using ammonium tartrate instead of sulfate salts, the new method preserves HIC performance while enabling compatibility with mass spectrometry.

• 3

  The developed rapid 10-minute HIC method allows for real-time monitoring of critical quality attributes in ADC bioconjugation reactions.

• 4

  Insights from the study revealed that the bioconjugation reaction was limited by dissolution rates, leading to significant process efficiency improvements.

• 5

  The findings emphasize the importance of real-time analytics in enhancing process understanding, efficiency, and product quality in pharmaceutical manufacturing.