5 Key Takeaways

• 1

  RNovA is a transformer-based framework for de novo peptide sequencing that identifies peptides and unexpected modifications from tandem mass spectra.

• 2

  The framework employs two modules, PathSearcher and SeqFiller, to analyze fragmentation paths and reconstruct full peptide sequences.

• 3

  In benchmark tests, RNovA outperformed existing methods, recovering multiple artificial modifications from complex biological samples.

• 4

  A year-long study of PM2.5 in Italy revealed significant seasonal and site-specific differences in organic aerosol composition.

• 5

  Mass spectrometry imaging of marine sediments demonstrated that sediment structure affects the reliability of climate signal recovery.