Analysis of Vitamin D Metabolites in Serum

contributed by Supelco | 09/05/2013

Improvement in LC-MS/MS Analysis by Leveraging Column Selectivity and Effective Sample Prep

Clinical interest in vitamin D stems from its implications in an ever-widening range of human health conditions.¹ This heightened interest has spawned the need for analytical strategies to assess an individual’s vitamin D status. Because LC-MS/MS has overcome many of the limitations of traditional immunoassay (e.g. lack of specificity, matrix interferences), it is becoming more and more the analytical method of choice for certain clinical assays, including vitamin D. To maximize the effectiveness of LC-MS/MS experiments, it is important to consider factors that reduce measurement sensitivity and accuracy, restrict throughput and cause instrument downtime. This brief report presents a rapid and sensitive LC-MS/MS method for accurate determination of 25-hydroxyvitamin D₂, 25-hydroxyvitamin D₃ and 3-epi-25-hydroxyvitamin D₃ in serum.

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Email*

Choose a password*

I have read and understand the Privacy Notice *

Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

Receive communication from The Cannabis Scientist I want to stay up to date with the Mass Spectrometry field I want to stay up to date with the Spectroscopy field

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.