Analysis of Vitamin D Metabolites in Serum

contributed by Supelco | 09/05/2013

Improvement in LC-MS/MS Analysis by Leveraging Column Selectivity and Effective Sample Prep

Clinical interest in vitamin D stems from its implications in an ever-widening range of human health conditions.¹ This heightened interest has spawned the need for analytical strategies to assess an individual’s vitamin D status. Because LC-MS/MS has overcome many of the limitations of traditional immunoassay (e.g. lack of specificity, matrix interferences), it is becoming more and more the analytical method of choice for certain clinical assays, including vitamin D. To maximize the effectiveness of LC-MS/MS experiments, it is important to consider factors that reduce measurement sensitivity and accuracy, restrict throughput and cause instrument downtime. This brief report presents a rapid and sensitive LC-MS/MS method for accurate determination of 25-hydroxyvitamin D₂, 25-hydroxyvitamin D₃ and 3-epi-25-hydroxyvitamin D₃ in serum.

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