Improve Reproducibility of MW Measurements of Polypeptoids using an EcoSEC GPC System

Summary

The EcoSEC GPC System and a set of TSKgel mixed-bed columns, both available from Tosoh Bioscience, were used successfully for obtaining high quality MWD data of a series of block poly-b-alkylalanoids with HFIP as the mobile phase in under 15 minutes. The apparent MW averages based of PMMA calibration ranged from 27,000 to 49,000 for M_n and from 30,000 to 61,000 for M_w with an average polydispersity of 1.20. Because of excellent flow rate and temperature control and baseline stability of the EcoSEC GPC System, average MW values ranged from 0.2 to 0.3% relative standard deviation, a ten-fold reproducibility improvement as compared to SEC literature data of polyamides using a similar mobile phase system.