Objective:

To reveal the stereochemical diversity of methionine oxidation in therapeutic antibodies, specifically distinguishing R and S forms.

Key Findings:

• NMR revealed multiple oxidation-dependent signals in antibodies.
• Met252 oxidizes more readily than Met428 due to its solvent exposure.
• The integrated approach allows visualization of structural differences at atomic resolution.

Interpretation:

The stereochemical analysis of methionine oxidation can significantly impact antibody stability and interactions, suggesting improvements in stability testing and manufacturing control.

Limitations:

• The study primarily focused on two specific methionines and may not generalize to all antibody types.
• Further research is needed to explore the implications of these findings in clinical settings.

Conclusion:

The new analytical strategy enhances understanding of antibody oxidation, potentially leading to better therapeutic antibody development.