5 Key Takeaways

• 1

  A new analytical strategy reveals subtle antibody damage from methionine oxidation in therapeutic antibodies.

• 2

  Researchers distinguished R and S stereoisomers of methionine sulfoxide in IgG1 antibodies using advanced techniques.

• 3

  The study focused on Met252 and Met428, whose oxidation affects antibody stability and receptor interactions.

• 4

  NMR and LC-MS confirmed the identities and abundances of oxidized methionine species in antibodies.

• 5

  The findings could enhance stability testing and manufacturing control for antibody therapeutics.